Yes, botox can make your eyes twitching. In daily life, eye twitches happen to many people and they can happen quite often too. But on the other hand, many people can do nothing to stop it and even don't know the cause of it. Then what is the cause of the eye twitches. Generally speaking, Eye twitches are caused by muscle spasms within the eyelids so it is commonly believed that eye twitches are somehow related to the nervous system. It means that in some cases eliminating stress or reducing physical strain to the eyes will reduce twitches. While, Botox injections can cause damage to the nerves which in turn causes severe muscle twitches around the eyes. If you have this problem, you should talk to your doctor before having any Botox treatments, or you may let it more serious and dangerous.